| Item type |
紀要論文 / Departmental Bulletin Paper_02(1) |
| 公開日 |
2018-11-23 |
| タイトル |
|
|
タイトル |
接合によりグルコアミラーゼ遺伝子STA1が発現したビール酵母の育種 |
| その他のタイトル |
|
|
その他のタイトル |
Construction of a brewing yeast expressing the glucoamylase gene STA1 by mating |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
主題 |
グルコアミラーゼ |
| キーワード |
|
|
主題 |
ビール酵母 |
| キーワード |
|
|
主題 |
接合 |
| キーワード |
|
|
主題 |
マルトオリゴ糖 |
| キーワード |
|
|
主題 |
香気成分 |
| キーワード |
|
|
主題 |
glucoamylase |
| キーワード |
|
|
主題 |
brewing yeast |
| キーワード |
|
|
主題 |
mating |
| キーワード |
|
|
主題 |
maltooligosaccharide |
| キーワード |
|
|
主題 |
flavor compound |
| 著者 |
尾形, 智夫
オガタ, トモオ
Ogata, Tomoo
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Standard brewing yeast cannot utilize larger oligomers or dextrins, which represent about 25% of wort sugars. A brewing yeast strain that could ferment these additional sugars to ethanol would be useful for producing low-carbohydrate diabetic or low-calorie beers. In this study, a brewing yeast strain that secretes glucoamylase was constructed by mating. The resulting Saccharomyces cerevisiae 278/113371 yeast was MATa/? diploid, but expressed the glucoamylase gene STA1. At the early phase of the fermentation test in malt extract medium, the fermentation rate of the diploid STA1 strain was slower than those of both the parent strain S. cerevisiae MAFF113371 and the reference strain bottom-fermenting yeast Weihenstephan 34/70. At the later phase of the fermentation test, however, the fermentation rate of the STA1 yeast strain was faster than those of the other strains. The concentration of ethanol in the culture supernatant of the STA1 yeast strain after the fermentation test was higher than those of the others. The concentration of all maltooligosaccharides in the culture supernatant of the STA1 yeast strain after the fermentation test was lower than those of the parent and reference strains, whereas the concentrations of flavor compounds in the culture supernatant were higher. These effects are due to the glucoamylase secreted by the constructed STA1 yeast strain. In summary, a glucoamylase-secreting diploid yeast has been constructed by mating that will be useful for producing novel types of beer owing to its different fermentation pattern and concentrations of ethanol and flavor compounds |
| 書誌情報 |
発行日 2018-03-31
|
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1343-8867 |
| 書誌レコードID |
|
|
収録物識別子タイプ |
NCID |
|
収録物識別子 |
AA11225201 |
| 資源タイプ |
|
|
内容記述タイプ |
Other |
|
内容記述 |
Thesis or Dissertation |
| 更新日 |
|
|
日付 |
2018-11-24 |
|
日付タイプ |
Created |
31〔解説・総説〕接合によりグルコアミラーゼ遺伝子STA1が発現したビール酵母の育種(P53-56)（尾形）.pdf (461.9 kB)
