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蛍光性低酸素誘導因子の相関分析
https://maebashi-it.repo.nii.ac.jp/records/21
https://maebashi-it.repo.nii.ac.jp/records/216217ed6d-1236-45bd-a2a2-db9e831acc2d
名前 / ファイル | ライセンス | アクション |
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05論文(野村)(修正後).pdf (395.6 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper_02(1) | |||||
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公開日 | 2015-11-10 | |||||
タイトル | ||||||
タイトル | 蛍光性低酸素誘導因子の相関分析 | |||||
その他のタイトル | ||||||
その他のタイトル | Fluorescence correlation analysis of GFP-labelled HIF-1α in stable | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題 | 低酸素誘導因子 | |||||
キーワード | ||||||
主題 | 蛍光相関分光法 | |||||
キーワード | ||||||
主題 | ブラウン運動 | |||||
キーワード | ||||||
主題 | 拡散方程式 | |||||
キーワード | ||||||
主題 | 拡散定数 | |||||
キーワード | ||||||
主題 | HIF-1α | |||||
キーワード | ||||||
主題 | Fluorescence correlation spectroscopy | |||||
キーワード | ||||||
主題 | Brownian motion | |||||
キーワード | ||||||
主題 | Diffusion equation | |||||
キーワード | ||||||
主題 | Diffusion coefficient | |||||
著者 |
田中, 悠樹
× 田中, 悠樹× 野村, 保友× タナカ, ユウキ× ノムラ, ヤストモ× Tanaka, Yuki× Nomura, Yasutomo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | HIF-1α, hypoxia induced factor-1αis regarded as a target for drug development in several diseases such as cancer. For high throughput screening of HIF-1α-targeted drug, we need to determine the activity sensitively and quantitatively. In the present study, we proposed a method of fluorescence correlation analysis on HIF-1α activation in which Co2+ treatment against stable transformants of GFP-labelled HIF-1α mimicked hypoxia. In fluorescence correlation spectroscopy, we observed fluorescence intensity fluctuation within a volume element which fluorescence molecules enter and exit because of the Brownian motion in solution or cytosol. When one-component model was used for the analysis, it was difficult to discriminate diffusion coefficient of active form of HIF-1α with inactive one. In two-component model, however, a fraction of slow moving component, GFP-labelled HIF-1α increased significantly when the transformants were exposed to Co2+. In the case of high throughput screening for HIF-1α-targeted drug with fluorescence correlation spectroscopy, we should use the fraction of the slower moving component to judge the activation. | |||||
書誌情報 | 発行日 2015-11-10 | |||||
更新日 | ||||||
日付 | 2017-03-27 | |||||
日付タイプ | Created |