| Item type |
紀要論文 / Departmental Bulletin Paper_02(1) |
| 公開日 |
2015-11-10 |
| タイトル |
|
|
タイトル |
蛍光性低酸素誘導因子の相関分析 |
| その他のタイトル |
|
|
その他のタイトル |
Fluorescence correlation analysis of GFP-labelled HIF-1α in stable |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
主題 |
低酸素誘導因子 |
| キーワード |
|
|
主題 |
蛍光相関分光法 |
| キーワード |
|
|
主題 |
ブラウン運動 |
| キーワード |
|
|
主題 |
拡散方程式 |
| キーワード |
|
|
主題 |
拡散定数 |
| キーワード |
|
|
主題 |
HIF-1α |
| キーワード |
|
|
主題 |
Fluorescence correlation spectroscopy |
| キーワード |
|
|
主題 |
Brownian motion |
| キーワード |
|
|
主題 |
Diffusion equation |
| キーワード |
|
|
主題 |
Diffusion coefficient |
| 著者 |
田中, 悠樹
野村, 保友
タナカ, ユウキ
ノムラ, ヤストモ
Tanaka, Yuki
Nomura, Yasutomo
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
HIF-1α, hypoxia induced factor-1αis regarded as a target for drug development in several diseases such as cancer. For high throughput screening of HIF-1α-targeted drug, we need to determine the activity sensitively and quantitatively. In the present study, we proposed a method of fluorescence correlation analysis on HIF-1α activation in which Co2+ treatment against stable transformants of GFP-labelled HIF-1α mimicked hypoxia. In fluorescence correlation spectroscopy, we observed fluorescence intensity fluctuation within a volume element which fluorescence molecules enter and exit because of the Brownian motion in solution or cytosol. When one-component model was used for the analysis, it was difficult to discriminate diffusion coefficient of active form of HIF-1α with inactive one. In two-component model, however, a fraction of slow moving component, GFP-labelled HIF-1α increased significantly when the transformants were exposed to Co2+. In the case of high throughput screening for HIF-1α-targeted drug with fluorescence correlation spectroscopy, we should use the fraction of the slower moving component to judge the activation. |
| 書誌情報 |
発行日 2015-11-10
|
| 更新日 |
|
|
日付 |
2017-03-27 |
|
日付タイプ |
Created |
05論文（野村）（修正後）.pdf (395.6 kB)
